Center for Bioimage Informatics at Carnegie Mellon University:Data

Data
The following data collections have been collected by Murphy Lab and can be found at Murphy Lab data site . They are also a part of the Protein Subcellular Location Image Database . Additional image collections being used in the NSF-sponsored ITR collaborative project between UCSB and CMU are available at UC Santa Barbara Center for Bio-Image Informatics site .
	2D CHO Available at: http://murphylab.web.cmu.edu/data The collection consists of fluorescence microscope images of Chinese Hamster Ovary cells using five different labels (anti-giantin, Hoechst 33258 (DNA), anti-lamp2, anti-nop4, anti-tubulin). The images were collected using wide-field microscopy and corrected to remove out-of-focus fluorescence using the nearest neighbor method.

	2D HeLa Available at: http://murphylab.web.cmu.edu/data This collection consists of fluorescence microscope images of HeLa cells using ten differet labels (DAPI, anti-ER, anti-giantin, anti-gpp130, anti-lamp2, anti-mitochondria, anti-nucleolin, phalloidin, anti-transferrin receptor, anti-tubulin). The images were collected using wide-field microscopy and corrected to remove out-of-focus fluorescence using the nearest neighbor method. Parallel images of DNA distributions are included.

	3D HeLa Available at: http://murphylab.web.cmu.edu/data This collection consists of fluorescence microscope for the same probes as in the 2D HeLa collection (except that Propidium iodide was used in place of DAPI). The images were collected using laser-scanning confocal microscopy. Parallel images of total DNA and total protein are included.

	3D HeLa-UCE Available at: http://murphylab.web.cmu.edu/data This collection was created by Dr. Jack Rohrer's group and consists of fluorescence microscope images of cells expressing GFP-tagged constructs of the mannose-6-phosphate uncovering enzyme (UCE). The images were collected using laser-scanning confocal microscopy following the same protocol as the 3D HeLa collection. A web interface showing typical images of each mutant and how their patterns are automatically grouped is available .

	3D 3T3 Available at: http://murphylab.web.cmu.edu/data This collection was collected in collaboration with Dr. Jonathan Jarvik and Dr. Peter Berget and consists of fluorescence microscope images of cell lines expressing GFP-tagged proteins. The cell lines were obtained by CD-tagging to produce internal GFP-fusions in random proteins. The images were collected using spinning disk confocal microscopy and only images of GFP fluorescence were collected. A web interface showing typical images of each cell line and how their patterns are automatically grouped is available .