Image Collections

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Jelena Kovacevic and Robert F. Murphy, Directors The 2D CHO collection consists of fluorescence microscope images of Chinese Hamster Ovary cells using five different labels (anti-giantin, Hoechst 33258 (DNA), anti-lamp2, anti-nop4, anti-tubulin). The images were collected using wide-field microscopy and corrected to remove out-of-focus fluorescence using the nearest neighbor method. The collection is available at http://murphylab.web.cmu.edu/data. The 2D HeLa collection consists of fluorescence microscope images of HeLa cells using ten differet labels (DAPI, anti-ER, anti-giantin, anti-gpp130, anti-lamp2, anti-mitochondria, anti-nucleolin, phalloidin, anti-transferrin receptor, anti-tubulin). The images were collected using wide-field microscopy and corrected to remove out-of-focus fluorescence using the nearest neighbor method. Parallel images of DNA distributions are included. The collection is available at http://murphylab.web.cmu.edu/data and as part of the Protein Subcellular Location Image Database. The 3D HeLa collection consists of fluorescence microscope for the same probes as in the 2D HeLa collection (except that Propidium iodide was used in place of DAPI). The images were collected using laser-scanning confocal microscopy. Parallel images of total DNA and total protein are included. The collection is available at http://murphylab.web.cmu.edu/data and as part of the Protein Subcellular Location Image Database. The 3D HeLa-UCE collection was created by Dr. Jack Rohrer's group and consists of fluorescence microscope images of cells expressing GFP-tagged constructs of the mannose-6-phosphate uncovering enzyme (UCE). The images were collected using laser-scanning confocal microscopy following the same protocol as the 3D HeLa collection. A web interface showing typical images of each mutant and how their patterns are automatically grouped is available.The collection is available at http://murphylab.web.cmu.edu/data and as part of the Protein Subcellular Location Image Database. The 3D 3T3 collection was collected in collaboration with Dr. Jonathan Jarvik and Peter Berget and consists of fluorescence microscope images of cell lines expressing GFP-tagged proteins. The cell lines were obtained by CD-tagging to produce internal GFP-fusions in random proteins. The images were collected using spinning disk confocal microscopy and only images of GFP fluorescence were collected. A web interface showing typical images of each cell line and how their patterns are automatically grouped is available. The collection is available at http://murphylab.web.cmu.edu/data and as part of the Protein Subcellular Location Image Database. Additional image collections being used in the NSF-sponsored ITR collaborative project between UCSB and CMU are available at http://www.bioimage.ucsb.edu/collections.html.